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after the autoclave sterilation

Discussion in 'Everything Else Orchid' started by robby jones, Oct 12, 2016.

  1. Oct 12, 2016 #1
    robby jones

    robby jones complete newby

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    ok so in a sterile cabinet you 100% clean everything,then put the mixed agar p668 solution into flasks n clean/put lids on the sterilize in auto clave.

    after that theyre hot obviously.

    do you let them cool before refridgerating for the agar to set ? or will it set at unrefridgerated room temp.

    after theyve set n sealed etc,how long before you clean the seed in 3% peroxide n put into flasks ? few days/weeks please ?
     
  2. Oct 12, 2016 #2
    Marni

    Marni Well-Known Member Staff Member Supporting Member

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    When you autoclave them, you leave the lid slightly loose so the pressure can equalize. You tighten them once they are cool enough to handle. The should gel at room temperature if everything is done properly. No need to put them in the refrigerator. Everything in the flask should be sterile. As soon as they are cooled to room temperature you can sow seed on them. With clean seed, 3% H2O2 needs to soak for at least 30 minutes with frequent agitation to work effectively. If the seed capsule was opening in the greenhouse or other growing environment you will need a longer time.
     
  3. Oct 13, 2016 #3
    robby jones

    robby jones complete newby

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    this is becoming very interresting indeed. id love to atleast try and germinate atleast once. n hopefully be successfull at it. not playing god ,however the process is within my limits i think....

    the p668 1 liter pack is posted ready to use please Marni ??? from the recomended aus dealer western orchids adelaide SA...im told he makes his own p668 but ive not ask him direct.

    Rod
     
  4. Oct 13, 2016 #4
    Marni

    Marni Well-Known Member Staff Member Supporting Member

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    Phytotech668 needs agar. You should ask the person you are buying it from.
     
  5. Oct 13, 2016 #5
    robby jones

    robby jones complete newby

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    its western orchids adelaide.he was recomended in the forum to me.it appears he makes his own version i think he said..when a newby starts i hear all sorst off things n thats good or sometimes its more confusing rofl.


    rod